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| This kit utilizes a biotinlyated secondary antibody to locate where the mouse primary antibody is bound to the
target antigen. Streptavidin-HRP (horseradish peroxidase) is then applied and binds to the secondary antibody.
This complex is observed through the use of a substrate-chromogen solution, which when added, results in a
colored precipitate at the antigen location. The staining location and pattern is easily observable by light
microscopy. |
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