Profiler array offers chemiluminescent detection for high-throughput gene expression profiling, so you can focus on your experiments instead of worrying about safety issues and costly radioactive disposal procedures.

FAST Exposure times
Profiler's digoxigenin-labeled probes are just as sensitive as radioactively labeled probes. And because exposure times are much shorter than with radioactive probes, you get results in minutes, not days.

Efficient & selective hybridization
We guarantee that each spot on the Star Profiler Array is free of homologous sequences, so you get efficient and selective hybridization with your labeled probe. Star Profiler is the only commercially available array made with long oligonucleotides, which we carefully design to make sure every sequence is unique.

Focus on key genes
The Profiler Array is a nylon membrane that contains genes that play significant roles in several pathways and are corroborated with published literature. We've specially designed Star Profiler to make high-throughput expression profiling of cancer or diseased tissues relatively easy. Star Profiler Array contains genes from six biologically significant groups: Oncogenes & Tumor Suppressors, Cell Cycle, Apoptosis, Kinases, Cytokines & Receptors, and Regulatory Proteins. In addition, we our developing targeted arrays devoted to specific areas of research. Currently we offer the Oncogene Profiler Array, which contains 295 human oncogenes and tumor suppressors. Other targeted arrays, such as the Apoptosis Profiler Array will be available in 2001.

Benefits of the Profiler Array
Unlike other macroarrays, which often require poly-A+ RNA, the Profiler Array has no preference for total or poly-A+ RNA. In fact, our method provides equivalent results using either total RNA or mRNA, so there is no need for special RNA purification procedures.

After cDNA labeling, Profiler Arrays are hybridized overnight with DIG-11-dUTP-labeled cDNA. The next day, the array membranes are washed and subsequently incubated with Anti-DIG-Alkaline Phosphatase (DIG-AP) Antibody. Upon addition of the CDP-Star chemiluminescent substrate (provided), detection of DIG-AP can be obtained within minutes by exposing array membranes to x-ray film (Figure 1 & 2) or by using a chemiluminescent-imaging system, such as Alpha Innotech's FluorChem Imaging System (Figure 3).

Each Profiler Array Nonradioactive System includes two membrane arrays; solutions for hybridization and washing, random primer and dNTP Mix for cDNA probe synthesis, and stripping; control RNA; and chemiluminescent detection reagents. Profiler arrays can be purchased separately and are provided with hybridization and array buffer, as well as washing, and stripping solutions. A complete gene list, including full names and GenBank accession numbers, are provided with each purchase.

Figures 2. Image of Star Profiler Array generated using Alpha Innotech's FluorChem Imager.